Recombinant Human PVR Protein

SKU: orb2994956

Description

Recombinant Human Poliovirus Receptor is produced by our Mammalian expression system and the target gene encoding Trp21-Asn343 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human CD96-His(Cat#orb2994268) at 2μg/ml (100 μl/well)can bind Human PVR-Fc(Cat#orb2994956).The ED50 of Human PVR-Fc(Cat#orb2994956) is 0.15 ug/ml. Immobilized Anti-Human PVR Antibody (NTX_bio, Research Grade)(Cat#orb2992528) at 2μg/ml (100 μl/well) can bind Recombinant Human PVR (C-Fc)(Cat#orb2994956)*. *: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human PVR (C-Fc)(Cat#orb2994956) is 2.11ng/ml.(Regularly tested)
• Tag: C-Fc
• Molecular Weight: Predicted: 62.2 KDa. Observed: 80-120 KDa, reducing conditions
• Expression Region: Trp21-Asn343
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Poliovirus Receptor; Nectin-Like Protein 5; NECL-5; CD155; PVR; PVS

Quick Database Links

UniProt

P15151