Recombinant Human Platelet-derived Growth Factor-AA

SKU: orb1906268

Description

Recombinant Human Platelet-derived Growth Factor-AA

Research Area

Stem Cell & Developmental Biology

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 50--200 ng/mL in a fluorometric assay using the redox sensitive dye.
• Molecular Weight: Approximately 28.8 kDa, a disulfide-linked homodimeric protein containing two 126 amino acid residues polypeptide. But it migrates with an apparent molecular mass of 33.6 kDa in SDS-PAGE.
• Protein Sequence: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
• Purity: > 95 % by SDS-PAGE.
• Endotoxins: Less than 0.1 EU/ug of rHuPDGF-AA as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only