Recombinant Human Platelet-derived Growth Factor-CC

SKU: orb1906263

Description

Recombinant Human Platelet-derived Growth Factor-CC

Research Area

Stem Cell & Developmental Biology

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 70-350 ng/ml.
• Molecular Weight: Approximately 27 kDa, a disulfide-linked homodimer of two 117 amino acid, C-terminal polyhistidine tagged proteins.
• Protein Sequence: Val235-Gly345, with an N-terminal Met and 6-His tag; Accession # NP_057289
• Purity: > 97 % by SDS-PAGE analyses.
• Endotoxins: Less than 0.1 EU/ug of rHuPDGF-CC as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only