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Recombinant Human PCSK9 Protein

SKU: orb2992748

Description

Recombinant Human Proprotein convertase subtilisin/kexin type 9 is produced by our Mammalian expression system and the target gene encoding Gln31-Gln692(Asp374Tyr) is expressed with a 6His tag at C-terminus.

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological OriginHuman
Biological ActivityELISA: Immobilized Anti-Human PCSK9 mAb (Human IgG1) at 2μg/ml (100 μl/well) can bind Recombinant Human PCSK9 V2 (D374Y,C-6His) (Cat#orb2992748). The ED50 of Recombinant Human PCSK9 V2 (D374Y,C-6His) (Cat#orb2992748) is 30.94 ng/ml. (Regularly tested). BLI: Loaded Anti-Human PCSK9 mAb (Human IgG1) on Pro-A Biosensor, can bind Recombinant Human PCSK9 V2 (D374Y,C-6His) (Cat#orb2992748) with an affinity constant of 2.77 nM as determined in BLI assay. (Regularly tested)
TagC-6His
Molecular WeightPredicted: 71.9 KDa. Observed: (17-21)&(60-70) KDa, reducing conditions
Expression RegionGln31-Gln692(Asp374Tyr)
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins< 1 EU/µg as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μm filtered solution of 20mM NaH2PO4, 150mM NaCl, 0.1M Arginine, 0.1M Glu, 0.01% Tween20, pH 7.4.
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Proprotein Convertase Subtilisin/Kexin Type 9; Neural Apoptosis-Regulated Convertase 1; NARC-1; Proprotein Convertase 9; PC9; Subtilisin/Kexin-Like Protease PC9; PCSK9; NARC1

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Recombinant Human PCSK9 Protein (orb2992748)

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