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Description
Research Area
Metabolism Research
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Anti-Human PCSK9 mAb (Human IgG1) at 2μg/ml (100 μl/well) can bind Recombinant Human PCSK9 V2 (D374Y,C-6His) (Cat#orb2992748). The ED50 of Recombinant Human PCSK9 V2 (D374Y,C-6His) (Cat#orb2992748) is 30.94 ng/ml. (Regularly tested). BLI: Loaded Anti-Human PCSK9 mAb (Human IgG1) on Pro-A Biosensor, can bind Recombinant Human PCSK9 V2 (D374Y,C-6His) (Cat#orb2992748) with an affinity constant of 2.77 nM as determined in BLI assay. (Regularly tested) |
| Tag | C-6His |
| Expression Region | Gln31-Gln692(Asp374Tyr) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| MW | Predicted: 71.9 KDa. Observed: (17-21)&(60-70) KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM NaH2PO4, 150mM NaCl, 0.1M Arginine, 0.1M Glu, 0.01% Tween20, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Proprotein Convertase Subtilisin/Kexin Type 9; Neural Apoptosis-Regulated Convertase 1; NARC-1; Proprotein Convertase 9; PC9; Subtilisin/Kexin-Like Protease PC9; PCSK9; NARC1
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−Recombinant Human PCSK9 V2 Protein [orb2992796]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 71.9 KDa. Observed: (15-19)&(60-80) KDa, reducing conditions
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Human PCSK9 Protein, hFc Tag [orb1173897]
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 97.2 kDa after removal of the signal peptide.The apparent molecular mass of PCSK9-hFc is approximately 100-130 kDa due to glycosylation.
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Recombinant human PCSK9 protein, C-His (HEK293) [orb1817204]
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Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide