Recombinant Human Osteopontin/OPN

SKU: orb1905934

Description

Recombinant Human Osteopontin/OPN

Research Area

Musculoskeletal & Connective Tissue Research

Key Properties

• Source: Mouse myeloma cell line, NS0
• Expression System: Mouse myeloma cell line, NS0
• Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x105 cells/well are added to a rHuOsteopontin/OPN coated plate, cell adhesion is enhanced in a dosedependent manner after 1 hour incubation at 37˚C. The ED50 for this effect is typically 0.1-0.6 μg/mL. rHuThrombin proteolytic treatment of this rHuOsteopontin can increase HEK293 cell adhesion by about 5-fold.
• Molecular Weight: Apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 290 amino acids including a C-terminal 6x polyhistidine tag.
• Protein Sequence: Ile17-Asn300, with a C-terminal 6-His tag; Accession # NP_000573.1
• Purity: > 95 % by SDS-PAGE analyses.
• Endotoxins: Less than 0.1 EU/ug of rHuOsteopontin/OPN as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from 0.2 μm filtered concentrated solution in PBS, pH 7.2.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only