Human Nectin-4

SKU: orb2993880

Description

Recombinant Human Poliovirus Receptor-Related Protein 4 is produced by our Mammalian expression system and the target gene encoding Gly32-Val351 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Anti-Human Nectin-4 mAb-mFc(Cat#orb2993141) at 2μg/ml (100 μl/well) can bind Human Nectin-4-Fc (Cat#orb2993880). The ED50 of Human Nectin-4-Fc (Cat#orb2993880) is 2.79 ng/ml. (Regularly tested). BLI: Loaded Anti-Human Nectin-4 mAb-mFc(Cat#orb2993141) on AMQ Biosensor, can bind Human Nectin-4-Fc(Cat#orb2993880) with an affinity constant of 0.86 nM as determined in BLI assay.
• Tag: C-Fc
• Molecular Weight: Predicted: 61.2 KDa. Observed: 75-90 KDa, reducing conditions
• Expression Region: Gly32-Val351
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR

Quick Database Links

UniProt

Q96NY8