Recombinant Human Nectin-1 Protein

SKU: orb2995040

Description

Recombinant Human Poliovirus Receptor-Related Protein 1 is produced by our Mammalian expression system and the target gene encoding Gln31-Thr334 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Human Nectin-4-mFc(Cat#orb2993807) on AMC Biosensor, can bind Human Nectin-1-His(Cat#orb2995040) with an affinity constant of 27.7 nM as determined in BLI assay. Loaded Human Nectin-4-Fc(Cat#orb2993880) on Protein A Biosensor, can bind Human Nectin-1-His(Cat#orb2995040) with an affinity constant of 24.18 nM as determined in BLI assay. Loaded Biotinylated Human Nectin-4-His-Avi(Cat#orb2993794) on SA Biosensor, can bind Human Nectin-1-His(Cat#orb2995040) with an affinity constant of 8.83 nM as determined in BLI assay.
• Tag: C-6His
• Molecular Weight: Predicted: 34.99 KDa. Observed: 40-60 KDa, reducing conditions
• Expression Region: Gln31-Thr334
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Poliovirus Receptor-Related Protein 1; Herpes Virus Entry Mediator C; Herpesvirus Entry Mediator C; HveC; Herpesvirus Ig-Like Receptor; HIgR; Nectin-1; CD111; PVRL1; HVEC; PRR1

Quick Database Links

UniProt

Q15223