Human NCF1

SKU: orb2994445

Description

Recombinant Human Neutrophil Cytosol Factor 1 is produced by our E.coli expression system and the target gene encoding Met1-Val390 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: E.coli
• Biological Origin: Human
• Tag: C-6His
• Molecular Weight: Predicted: 45.6 KDa. Observed: 45-50 KDa, reducing conditions
• Expression Region: Met1-Val390
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

NCF-1; 47 kDa autosomal chronic granulomatous disease protein/47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2/SH3 and PX domain-containing protein 1A; p47-phox

Quick Database Links

UniProt

P14598