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Catalog Number | orb1906239 |
---|---|
Category | Proteins |
Description | Recombinant Human Midkine |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Purity | > 97 % by SDS-PAGE and HPLC analyses. |
MW | Approximately 13.4 kDa, a single non-glycosylated polypeptide chain containing 123 amino acids. |
UniProt ID | P21741 |
Protein Sequence | VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD |
Source | Escherichia coli |
Expression System | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml. |
Endotoxins | Less than 1 EU/ug of rHuMidkine as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Alternative names | ARAP, Midgestation and Kidney Protein, Neurite Out Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 39.6 kDa after removal of the signal peptide. The apparent molecular mass of MDK-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 39.4 kDa after removal of the signal peptide. The apparent molecular mass of cMDK-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
40.4 kDa | |
E.coli |
> 97% as determined by SDS-PAGE and HPLC. | |
13.4 kDa | |
E.Coli |
SDS-PAGE | |
Unconjugated | |
> 97% by SDS-PAGE and HPLC analyses. | |
13.4 kDa | |
E. coli |