Recombinant Human MICA Protein

SKU: orb2995041

Description

Recombinant Human MHC Class I Polypeptide-Related Sequence A is produced by our Mammalian expression system and the target gene encoding Glu24-Gln308 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Human NKG2D-Fc(Cat#orb2994116) on Protein A Biosensor, can bind Human MICA-His(Cat#orb2995041) with an affinity constant of 24.6 nM as determined in BLI assay. (Regularly tested)
• Tag: C-6His
• Molecular Weight: Predicted: 33.87 KDa. Observed: 60 KDa, reducing conditions
• Expression Region: Glu24-Gln308
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MHC Class I Polypeptide-Related Sequence A; MIC-A; MICA; PERB11.1

Quick Database Links

UniProt

AAH16929.1