Human Mesothelin

SKU: orb2994280

Description

Recombinant Human Mesothelin is produced by our Mammalian expression system and the target gene encoding Glu296-Ser598 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human Mesothelin (C-6His) (Cat#orb2994280) at 2 μg/ml (100 μl/well) can bind Anti-Human Mesothelin mAb (Cat#orb2992719). The ED50 of Anti-Human Mesothelin mAb (Cat#orb2992719) is 4.88 ng/ml. (Regularly tested). BLI: Loaded Anti-Human Mesothelin mAb-Fc(Cat#orb2992719) on Protein A Biosensor, can bind Human Mesothelin-His(Cat#orb2994280) with an affinity constant of 0.1 nM as determined in BLI assay.
• Tag: C-6His
• Molecular Weight: Predicted: 34.9 KDa. Observed: 35-50 KDa, reducing conditions
• Expression Region: Glu296-Ser598
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20 mM Hepes-NaOH, 50 mM NaCl, 8% Trehalose, 0.05% Tween 80, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Megakaryocyte potentiating factor; mesothelin; Pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein; CAK1; MPF; MSLN; SMR; CAK1; CAK1 antigen

Quick Database Links

UniProt

AAH09272.1