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Recombinant Human Melanoma Inhibitory Activity Protein
SKU: orb1905941
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Description
Research Area
Cancer Biology
Images & Validation
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Key Properties
−| Source | Escherichia coli |
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| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 μg/ml, corresponding to a specific activity of > 200 IU/mg. |
| Endotoxins | Less than 0.1 EU/ug of rHuMIA as determined by LAL method. |
| MW | Approximately 12.1 kDa, a single non-glycosylated polypeptide chain containing 107 amino acids. |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
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| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Recombinant Human MIA Protein [orb2995217]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 13.31 KDa. Observed: 14 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg- Item 1 of 2
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