Recombinant Human LY75 Protein

SKU: orb2992705

Description

Recombinant Human Lymphocyte antigen 75 is produced by our Mammalian expression system and the target gene encoding Ser28-Asp1666 is expressed with a human IgG1 Fc tag at the C-terminus.

Research Area

Immunology & Inflammation

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human LY75 (C-Fc) (Cat#orb2992705) at 5 μg/ml (100 μl/well) can bind Anti-Human CD205 mAb (Human IgG1)*. *:Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human CD205 mAb (Human IgG1) is 4.282 ng/ml. (Regularly tested).
• Tag: C-Fc
• Molecular Weight: Predicted: 215.7 KDa. Observed: 175-260 KDa, reducing conditions
• Expression Region: Ser28-Asp1666
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: ＜ 1 EU/μg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lymphocyte antigen 75; Ly-75; C-type lectin domain family 13 member B; CD205; LY75; CLEC13B

Quick Database Links

UniProt

O60449