Recombinant Human Liver carboxylesterase 1 (CES1) (Active)

SKU: orb2658694

Description

This Recombinant Human Liver carboxylesterase 1 (CES1) spans the amino acid sequence from region 18-567aa. Purity: Greater than 95% as determined by SDS-PAGE.

Research Area

Gastroenterology & Hepatology

Images & Validation

• Application Notes:
  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

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Key Properties

• Source: Mammalian cell
• Biological Origin: Homo sapiens (Human)
• Biological Activity: Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is >40000 pmol/min/µg.
• Tag: C-terminal 10xHis-tagged
• Expression Region: 18-567aa
• Protein Length: Full Length of Mature Protein
• Endotoxins: Less than 1.0 EU/μg as determined by LAL method.
• MW: 62.0 kDa
• Purity: Greater than 95% as determined by SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
• Protein Sequence: GHPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMQLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHIEL

Storage & Handling

• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Form/Appearance: Lyophilized powder
• Buffer/Preservatives: Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6%Trehalose, pH 8.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Liver carboxylesterase 1; Acyl-coenzyme A:cholesterol acyltransferase (ACAT); Brain carboxylesterase hBr1; Carboxylesterase 1 (CE-1; hCE-1) (EC:3.1.1.13 ) ; Cholesteryl ester hydrolase 1 publication (CEH 1 publication) (EC:3.1.1.134); Cocaine carboxylesterase;CES1 ；CES2, SES1

Quick Database Links

UniProt

P23141