Human LIGHT

SKU: orb2994330

Description

Recombinant Human TNF Ligand Superfamily Member 14 is produced by our Mammalian expression system and the target gene encoding Leu83-Val240 is expressed with a 6His tag at the N-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human LIGHT (N-6His)(Cat#orb2994330) at 5μg/ml (100 μl/well) can bind Recombinant Rhesus Macaque HVEM (C-Fc)(Cat#orb2994821).The ED50 of Recombinant Rhesus Macaque HVEM (C-Fc)(Cat#orb2994821) is 17.51 ng/ml. (Regularly tested). BLI: Loaded Mouse LTBR-Fc(Cat#orb2993964) on Protein A Biosensor, can bind Human LIGHT-His(Cat#orb2994330) with an affinity constant of 0.81 nM as determined in BLI assay. (Regularly tested)
• Tag: N-6His
• Molecular Weight: Predicted: 18.2 KDa. Observed: 18-24 KDa, reducing conditions
• Expression Region: Leu83-Val240
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tumor necrosis factor ligand superfamily member 14; Herpes virus entry mediator ligand; TNFSF14; HVEM-L; LIGHT

Quick Database Links

UniProt

AAC39563.1