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Description
Research Area
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Recombinant Human LILRB4 (Cat#orb2994032) on Pro-A Biosensor, can bind Recombinant Human LGALS8 (Cat#orb2995237) with an affinity constant of 0.629 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human LILRB4 (Cat#orb2994031) on HIS1K Biosensor, can bind Recombinant Human LGALS8(Cat#orb2995237) with an affinity constant of 0.389 uM as determined in BLI assay. (Regularly tested) |
| Tag | Tag free |
| Expression Region | Met1-Trp317 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 35.8 KDa. Observed: 32 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Protocol Information
Recombinant Human LGALS8 Protein (orb2995237)
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