Recombinant Human Leptin

• Catalog Number: orb1905976
• Category: Proteins
• Description: Recombinant Human Leptin
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20.
• Purity: > 97 % by SDS-PAGE and HPLC analyses.
• MW: Approximately 16.0 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
• UniProt ID: P41159
• Protein Sequence: VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC
• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x105 IU/mg.
• Endotoxins: Less than 1 EU/ugof rHuLeptin as determined by LAL method.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Alternative names: Obese Protein, Obesity Factor
• Note: For research use only
• Expiration Date: 6 months from date of receipt.