Recombinant Human LAG-3 Protein

SKU: orb2994123

Description

Recombinant Human Lymphocyte Activation Gene 3 Protein is produced by our Mammalian expression system and the target gene encoding Leu23-Leu450 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human LAG-3-His (Cat#orb2994123) at 5μg/ml (100 μl/well) can bind Human FGL1-mFc (Cat#orb2994023).The ED50 of Recombinant Human FGL1-mFc (Cat#orb2994023) is 30-90 ng/ml.. BLI: Loaded Human FGL1-Fc(Cat#orb2993935) on Protein A Biosensor, can bind Human LAG-3-His(Cat#orb2994123) with an affinity constant of 88.12nM as determined in BLI assay. Loaded Anti-Human LAG-3 mAb-Fc(Cat#Norb3002363) on Protein A Biosensor, can bind Human LAG-3-6His(Cat#orb2994123) with an affinity constant of 0.41 nM as determined in BLI assay.
• Tag: C-6His
• Molecular Weight: Predicted: 47.2 KDa. Observed: 60-80 KDa, reducing conditions
• Expression Region: Leu23-Leu450
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, 2mM EDTA, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lymphocyte activation gene 3 protein; LAG3; LAG-3; Protein FDC; CD223

Quick Database Links

UniProt

P18627