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Catalog Number | orb1631645 |
---|---|
Category | Proteins |
Description | Recombinant Human Interleukin-6(rHu IL-6) |
Form/Appearance | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
Purity | > 96 % by SDS-PAGE and HPLC analyses. |
MW | Approximately 20.7 kDa, a single non-glycosylated polypeptide chain containing 183 amino acids. |
UniProt ID | P05231 |
Source | Human |
Expression System | Escherichia coli. |
Biological Activity | Assay #1: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine T1165 cells is less than 0.8 ng/ml, corresponding to a specific activity of > 1.25 × 106 IU/mg. |
Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
Alternative names | BSF-2, CDF, Hybridoma growth factor, IFN-beta-2. Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |