Recombinant Human Interleukin-20 (rHuIL-20)

SKU: orb1495041

Description

IL-20 is a member of the IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences but they are dissimilar in their biological functions. IL-20 is a hematopoietic growth factor capable of stimulating colony formation by CD34+ multipotential progenitors, but not by other progenitor cells. IL-20 signals through a receptor system composed of type I IL-20R-α and type II IL-20R-β. Over-expression of IL-20 in keratinocytes expressing both receptor subunits has been implicated in the induction of inflammatory skin disease.

Images & Validation

• Application Notes:
  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Key Properties

• Source: Escherichia coli.
• Biological Activity: Measured by its ability to activate STAT following receptor ligand interaction, it’s fully biologically active when compared to standard.
• Molecular Weight: Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
• Protein Sequence: MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE
• Purification: >95% by SDS-PAGE and HPLC analyses.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 1EU/mg of rHuIL-20 as determined by LAL method.

Storage & Handling

• Storage: This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Form/Appearance: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
• Buffer/Preservatives: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
• Disclaimer: For research use only