Recombinant Human Insulin-like Growth Factor-Binding Protein 4, Insect Cells Derived

SKU: orb1906278

Description

Recombinant Human Insulin-like Growth Factor-Binding Protein 4, Insect Cells Derived

Research Area

Metabolism Research; Stem Cell & Developmental Biology

Key Properties

• Source: Insect Cell
• Expression System: Insect Cell
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.1 μg/ml, corresponding to a specific activity of > 1.0 x104 IU/mg in the presence of 14 ng/ml of rHuIGF-II.
• Molecular Weight: The apparent molecular weight is about 30 kDa in SDS-PAGE under reducing conditions, containing 237 amino acids.
• Protein Sequence: DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE
• Purity: > 97 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 0.1 EU/ug of rHuIGF-BP4, Insect Cell as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in 20mM Tris-HCl, pH 8.0, 150mM NaCl.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only