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Catalog Number | orb1099631 |
---|---|
Category | Proteins |
Description | Recombinant Human IL6R |
Tested applications | ELISA, WB |
Reactivity | Human |
Tag | N-terminal His-IF2DI Tag |
Purity | Greater than 95% by SDS-PAGE gel analyses |
MW | 47.9kDa |
UniProt ID | P08887 |
Source | E.Coli |
Expression Region | 229-468 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH 7.4 |
Alternative names | CD 126, CD126, CD126 antigen, gp80, IL 6R, IL 6R 1 Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 39.4 kDa after removal of the signal peptide.The apparent molecular mass of IL6R-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 64.7 kDa after removal of the signal peptide.The apparent molecular mass of IL6R-hFc is approximately 70-100 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 94% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 65-78 kDa based on Tris-Bis PAGE result. |
> 95% as determined by Tris-Bis PAGE; > 94% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |