Human IL-4 RA

• Catalog Number: orb2994196
• Category: Proteins
• Description: Recombinant Human Interleukin-4 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Met26-Gln231 is expressed with a human IgG1 Fc tag at the C-terminus.
• Conjugation: Unconjugated
• Tag: C-Fc
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• UniProt ID: P24394
• MW: Predicted: 50.2 KDa. Observed: 60-70 KDa, reducing conditions
• Expression System: Human Cells
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human IL-4 RA-Fc(Cat#orb2994196) at 5μg/ml (100 μl/well)can bind Human IL-4 *(Cat#orb3002477) .*: Biotinylated by NHS-biotin prior to testing.The ED50 of Human IL-4 (Cat#orb3002477)is 2.43 ng/ml.. BLI: Loaded Human IL-4-His(Cat#orb3002435) on HIS1K Biosensor, can bind Human IL-4RA-Fc(Cat#orb2994196) with an affinity constant of 0.12 nM as determined in BLI assay. . Cell Based Assay: Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells.The ED50 for this effect is 5-20 ng/ml. (QC verified)
• Expression Region: Met26-Gln231
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Alternative names: Interleukin-4 receptor subunit alpha; IL-4 recepto
• Note: For research use only
• Expiration Date: 6 months from date of receipt.