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| Catalog Number | orb2994461 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human Interleukin-2 Receptor Subunit Beta is produced by our Mammalian expression system and the target gene encoding Ala27-Asp239 is expressed with a human IgG1 Fc tag at the C-terminus. |
| Conjugation | Unconjugated |
| Tag | C-Fc |
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| UniProt ID | P14784 |
| MW | Predicted: 51.7 KDa. Observed: 60-80 KDa, reducing conditions |
| Expression System | Human Cells |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Biotinylated Human IL-2-His-Avi(Cat#orb2993791) on SA Biosensor, can bind Human IL-2RB-Fc(Cat#orb2994461) with an affinity constant of 12.62nM as determined in BLI assay. |
| Expression Region | Ala27-Asp239 |
| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| Alternative names | Interleukin-2 receptor subunit beta;IL2RB;IL-2 rec Read more... |
| Note | For research use only |
Biotin | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 27.4 KDa. Observed: 35-45 KDa, reducing conditions |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 52.2 KDa. Observed: 70-110 KDa, reducing conditions |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) | |
Predicted: 54&51 KDa. Observed: (57-72)&(85-115) KDa, reducing conditions |
Unconjugated | |
SEC-HPLC: Greater than 90% as determined by SEC-HPLC.(QC verified) | |
Predicted: 25.6 KDa. Observed: 35-40 KDa, reducing conditions |
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