Human IL-23

• Catalog Number: orb2993911
• Category: Proteins
• Description: Recombinant Human Interleukin-23 is produced by our Mammalian expression system and the target gene encoding Arg20-Pro189&Ile23-Ser328 is expressed with a 6His tag at the C-terminus.
• Conjugation: Unconjugated
• Tag: C-6His
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM Trehalose, 4% Mannitol, 50mM NaCl, 0.02% Tween80, pH 7.5.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)
• UniProt ID: Q9NPF7, P29460
• MW: Predicted: 19.7&34.8 KDa. Observed: 19-21&40-60 KDa, reducing conditions
• Expression System: Human Cells
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human IL-23R-Fc(Cat#orb2994313) at 10μg/ml (100 μl/well)can bind Human IL-23-His(Cat#orb2993911).The ED50 of Human IL-23-His(Cat#orb2993911) is 0.248 ug/ml.. BLI: Loaded Ustekinumab on Pro-A Biosensor, can bind Recombinant Human IL-23 (C-6His) (Cat#orb2993911) with an affinity constant of 40.7 pM as determined in BLI assay. (Regularly tested) Loaded Human IL-23R-Fc(Cat#orb2994313) on Protein A Biosensor, can bind Human IL-23-His(Cat#orb2993911) with an affinity constant of 0.98 nM as determined in BLI assay. Loaded Anti-Human IL23-P19 mAb-Fc(Cat#orb2993116) on Protein A Biosensor, can bind Human IL-23-His(Cat#orb2993911) with an affinity constant of 2.2 pM as determined in BLI assay. . Cell Based Assay: Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 70-210 ng/ml. (QC verified)
• Expression Region: Arg20-Pro189&Ile23-Ser328
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Alternative names: SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2
• Note: For research use only