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Human IGHE
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human IGHE (104-427, C-6His) (Cat#orb2992856) at 2 μg/ml (100 μl/well) can bind Human IGHE Antibody (Omalizumab, Research Grade) (Cat#orb2992563). The ED50 of Human IGHE Antibody (Omalizumab, Research Grade) (Cat#orb2992563) is 5.39 ng/ml. (Regularly tested). |
| Tag | C-6His |
| Molecular Weight | Predicted: 37.1 KDa. Observed: 41-58 KDa, reducing conditions |
| Expression Region | Cys104-Lys427 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human IGHE [orb2992855]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 25.7 KDa. Observed: 28-38 KDa, reducing conditions
1 mg, 500 μg, 50 μg, 10 μgHuman IgE(Immunoglobulin E) Microsample ELISA Kit [orb2998826]
Human
1.57-100 µg/mL
0.65 µg/mL
48 T, 96 TRecombinant Human IgE [orb1098738]
ELISA, WB
Greater than 90% by SDS-PAGE gel analyses
36 kDa
HEK293 cells
50 μg, 100 μg, 200 μg, 1 mgHuman IGHE Protein, His Tag [orb1743299]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 47.8 kDa after removal of the signal peptide.
E.coli
10 μg, 50 μg, 100 μg

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Protocol Information
Human IGHE (orb2992856)
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