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Recombinant Human I-309/CCL1
Description
Images & Validation
−Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml. |
| Molecular Weight | Approximately 8.6 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids. |
| Protein Sequence | SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1 EU/ug of rHuI-309/CCL1 as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human CCL1 [orb3002330]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 8.62 KDa. Observed: 10 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman CCL1 Protein [orb1471777]
Unconjugated
Greater than 95% as determined by reducing SDS-PAGE.
8.62 KDa
Mammalian
50 μg, 10 μgRecombinantI-309/CCL1,Human [orb1494846]
> 95% as analyzed by SDS-PAGE.
8.5 kDa, observed by reducing SDS-PAGE.
Escherichia coli.
5 μg, 25 μgRecombinantI-309/CCL1,Human(CHO-expressed) [orb1494727]
> 98% as analyzed by SDS-PAGE.
15 kDa, observed by reducing SDS-PAGE.
CHO
10 μg, 50 μgRecombinant Human I-309/CCL1 (rHuI-309/CCL1) [orb1495050]
>97% by SDS-PAGE and HPLC analyses.
8.5 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids.
Escherichia coli.
2 μg, 10 μg, 1 mg

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UniProt Details
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Protocol Information
Recombinant Human I-309/CCL1 (orb1906073)
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