Human HLA-A*0201 AFP complex Protein

SKU: orb2993060

Description

Recombinant Human HLA-A*0201 AFP complex Protein is produced by our Mammalian expression system and the target gene encoding (FMNKFIYEI)&Ile21-Met119&Gly25-Ile308 is expressed with a 10His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Anti-Human AFP mAb-Fc(Cat#orb2992904) on Protein A Biosensor, can bind HLA-A*0201 AFP complex Protein-His(Cat#orb2993060) with an affinity constant of 1.6 nM as determined in BLI assay.
• Tag: C-10His
• Molecular Weight: Predicted: 49.2 KDa. Observed: 55-60 KDa, reducing conditions
• Expression Region: (FMNKFIYEI)&Ile21-Met119&Gly25-Ile308
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

NA

Quick Database Links

UniProt

P61769, P01892