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Catalog Number | orb1906266 |
---|---|
Category | Proteins |
Description | Recombinant Human Heparin-binding EGF-like Growth Factor |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, 500 mM NaCl, 5% trehalose, pH 7.4. |
Purity | > 97% by SDS-PAGE and HPLC analyses. |
MW | Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids. |
UniProt ID | Q99075 |
Source | Escherichia coli |
Expression System | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 x106 U/mg. |
Endotoxins | Less than 0.1 EU/ug of rHuHB-EGF as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Alternative names | HBEGF, DT-R Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Greater than 95% as determined by reducing SDS-PAGE. | |
15.1 KDa | |
Mammalian |
98.00% | |
16-27 KDa (reducing condition) |
Greater than 85% as determined by SDS-PAGE. | |
Escherichia Coli |