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Human GLP1R
SKU: orb2994095
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human GLP-1R (C-Fc) (Cat#orb2994095) at 5μg/ml (100 μl/well) can bind Anti-GLP-1R Antibody (Human IgG1). The ED50 of Anti-GLP-1R Antibody (Human IgG1) is 6.23 ng/ml.. BLI: Loaded Anti-GLP-1R Antibody(Human IgG1) on SA Biosensor, can bind Recombinant Human GLP1R (Arg24-Tyr145) (C-Fc)(Cat#orb2994095) with an affinity constant of 11.6 nM as determined in BLI assay. (Regularly tested) |
| Tag | C-Fc |
| Molecular Weight | Predicted: 41.2 KDa. Observed: 50-75 KDa, reducing conditions |
| Expression Region | Arg24-Tyr145 |
| Purity | SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Glucagon-like peptide 1 receptor; GLP-1 receptor; GLP-1-R
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Human GLP1R Protein, hFc Tag [orb1743290]
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 39.7 kDa after removal of the signal peptide. The apparent molecular mass of GLP1R-hFc is approximately 35-70 kDa due to glycosylation.
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Human GLP1R (orb2994095)
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