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Human GIPR N-ECD
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Anti-Human GIPR Antibody(L7H6) at 5μg/ml (100 μl/well) can bind Recombinant Human GIPR N-ECD(C-6His)(Cat#orb2992967). The ED50 of Recombinant Human GIPR N-ECD(C-6His)(Cat#orb2992967) is 5.60 ng/ml. (Regularly tested). BLI: Loaded Anti-Human GIPR Antibody(L7H6) on Pro-A Biosensor, can bind Recombinant Human GIPR N-ECD (C-6His) (Cat#orb2992967) with an affinity constant of 0.532 nM as determined in BLI assay. (Regularly tested) |
| Tag | C-6His |
| Molecular Weight | Predicted: 14.3 KDa. Observed: 17-30 KDa, reducing conditions |
| Expression Region | Arg22-Gln138 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 95% as determined by SEC-HPLC.(QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Predicted: 16.1 kDa. Observed: 20-30 kDa
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Unconjugated
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Unconjugated
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Human GIPR N-ECD (orb2992967)
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