Recombinant Human GCGR N-ECD Protein

SKU: orb2992951

Description

Recombinant Human Glucagon receptor is produced by our Mammalian expression system and the target gene encoding Ala26-Lys136 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human GCGR N-ECD (C-Fc) (Cat#orb2992951) at 10 μg/ml (100 μl/well) can bind Human GCGR Antibody (Volagidemab)(Cat#orb2992815). The ED50 of Human GCGR Antibody (Volagidemab)(Cat#orb2992815) is 65.57 ng/ml. (Regularly tested). BLI: Loaded Human GCGR Antibody (Volagidemab) (Cat#orb2992815) on SA Biosensor, can bind Recombinant Human GCGR N-ECD (C-Fc) (Cat#orb2992951) with an affinity constant of 0.581 nM as determined in BLI assay. (Regularly tested)
• Tag: C-Fc
• Molecular Weight: Predicted: 40 kDa. Observed: 47-75 kDa
• Expression Region: Ala26-Lys136
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glucagon receptor; GCGR; GL-R

Quick Database Links

UniProt

P47871