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Description
Research Area
Images & Validation
−
Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human AXL-His(Cat#orb2993857) at 5μg/ml (100 μl/well) can bind Human GAS6-Fc(Cat#orb2993048). The ED50 of Human GAS6-Fc(Cat#orb2993048) is 55.16 ng/ml. Immobilized Human Dtk-His(Cat#orb2994131) at 10μg/ml (100 μl/well) can bind Human GAS6-Fc(Cat#orb2993048). The ED50 of Human GAS6-Fc(Cat#orb2993048) is 0.336 ug/ml.. BLI: Loaded Mouse AXL-His(Cat#orb2993853) on HIS1K Biosensor, can bind Human GAS6-Fc(Cat#orb2993048) with an affinity constant of 0.006 nM as determined in BLI assay. Loaded Human Dtk-His(Cat#orb2994131) on HIS1K Biosensor, can bind Human GAS6-Fc(Cat#orb2993048) with an affinity constant of 0.02 nM as determined in BLI assay. |
| Tag | C-Fc |
| Expression Region | Ala31-Ala678 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 98.8 KDa. Observed: 110-120 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Recombinant Human GAS6 Protein (orb2993048)
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