Recombinant Human Galectin-1

Catalog Number: orb1905937

Catalog Number	orb1905937
Category	Proteins
Description	Recombinant Human Galectin-1
Form/Appearance	Lyophilized from a 0.2 μm filtered solution in PBS pH 7.0, 3mM β-Me, 5% Trehalose, 0.02% Tween 20.
Purity	> 95 % by SDS-PAGE and HPLC analyses.
MW	Approximately 14.6 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids.
UniProt ID	P09382
Protein Sequence	ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD
Source	Escherichia coli
Expression System	Escherichia coli
Biological Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human blood monocytes is in a concentration range of 1.0-10 ng/ml.
Endotoxins	Less than 0.1 EU/ug of rHuGalectin-1 as determined by LAL method.
Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Note	For research use only
Expiration Date	6 months from date of receipt.

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 40.7 kDa after removal of the signal peptide.The apparent molecular mass of LGALS1-hFc is approximately 35-55 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 100 μg, 10 μg, 50 μg

Purity:	Greater than 85% as determined by SDS-PAGE.
MW:	19.6 kDa
Source:	E.coli

Purity:	Greater than 90% as determined by SDS-PAGE.
MW:	30.6 kDa
Source:	E.coli

Applications:	ELISA, MS, SDS-PAGE, WB
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Source:	E. coli