Human Frizzled-7

SKU: orb2992571

Description

Recombinant Human Frizzled-7 is produced by our Mammalian expression system and the target gene encoding Gln33-Leu185 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human Frizzled-7 N-ECD (C-6His) (Cat#orb2992571) at 5 μg/ml (100 μl/well) can bind Human Frizzled receptors Antibody (Vantictumab, Research Grade)(Cat#orb2992588). The ED50 of Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) is 10.57 ng/ml. (Regularly tested).. BLI: Loaded Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) on Pro-A Biosensor, can bind Recombinant Human Frizzled-7 N-ECD (Cat#orb2992571) with an affinity constant of 0.346 uM as determined in BLI assay. (Regularly tested)
• Tag: C-6His
• Molecular Weight: Predicted: 17.5 KDa. Observed: 23-35 KDa, reducing conditions
• Expression Region: Gln33-Leu185
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: ＜ 1 EU/μg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Frizzled-7; FZD7

Quick Database Links

UniProt

O75084