Recombinant Human Frizzled-5 Protein

SKU: orb2992767

Description

Recombinant Human Frizzled-5 is produced by our Mammalian expression system and the target gene encoding Ala27-Pro167 is expressed with a 6His tag at C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human Frizzled-5 (C-6His) (Cat#orb2992767) at 2μg/ml (100 μl/well) can bind Anti-FZD5 mAb (Vantictumab). The ED50 of Anti-FZD5 mAb (Vantictumab) is 5.535 ng/ml. (Regularly tested)
• Tag: C-6His
• Molecular Weight: Predicted: 16.8 KDa. Observed: 20- 35 KDa, reducing conditions
• Expression Region: Ala27-Pro167
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Frizzled-5; FZD5; hFz5; Fz-5; Fzd5

Quick Database Links

UniProt

Q13467