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Description
Research Area
Neuroscience
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human FOLR1-His(Cat#orb2994909) at 1μg/ml (100 μl/well)can bind Anti-Human FOLR1 mAb(Cat#orb2993067).The ED50 of Anti-Human FOLR1 mAb(Cat#orb2993067)is 0.626 ng/ml.. BLI: Loaded Anti-Human FOLR1 mAb-Fc(Cat#orb2993067) on Protein A Biosensor, can bind Human FOLR1-His(Cat#orb2994909) with an affinity constant of 12.1 nM as determined in BLI assay. |
| Tag | C-6His |
| Expression Region | Arg25-Ser234 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 25.7 KDa. Observed: 28-38 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Folate receptor alpha;FR-alpha;Adult folate-binding protein;FBP;Folate receptor 1;Folate receptor;Ovarian tumor-associated antigen MOv18;FOLR1
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500 μg, 1 mg, 10 μg, 50 μgRecombinant Human FOLR1 Protein (Biotin) [orb2993862]
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Predicted: 27.5 KDa. Observed: 35-40 KDa, reducing conditions
20 μg, 100 μg- Item 1 of 2
- Item 1 of 2
Human FOLR2 Protein, His Tag [orb1173809]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 25.4 kDa after removal of the signal peptide. The apparent molecular mass of FOLR2-His is approximately 25-35 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μg
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Quick Database Links
UniProt
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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