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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x106 IU/mg. |
| Endotoxins | Less than 1 EU/ug of rHuFGF-4 as determined by LAL method. |
| MW | Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids. |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, with 350 mM NaCl, 5% trehalose and 0.02% Tween-20, pH 7.0. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HST, HST-1, HSTF-1, HBGF-4, Transforming Protein KS3
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Protocol Information
Protein Handling and Storage Guide
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