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Catalog Number | orb1906288 |
---|---|
Category | Proteins |
Description | Recombinant Human Fibroblast Growth Factor 9 |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Purity | > 95 % by SDS-PAGE and HPLC analyses. |
Protein Sequence | APLGEVGNYF GVQDAVPFGN VPVLPVDSPV LLSDHLGQSE AGGLPRGPAV TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELY KDILSQS |
UniProt ID | P31371 |
MW | Approximately 23.3 kDa, a single non-glycosylated polypeptide chain containing 207 amino acids. |
Expression System | Escherichia coli |
Endotoxins | Less than 1 EU/ug of rHuFGF-9 as determined by LAL method. |
Source | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x106 IU/mg. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Alternative names | GAF, Glia-activating factor, HBGF-9 |
Note | For research use only |
Greater than 95% as determined by SDS-PAGE. | |
23.44 kDa | |
E.coli |
Greater than 95% as determined by reducing SDS-PAGE. | |
23.44 KDa | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
Greater than 95% as determined by SDS-PAGE. | |
23.2 kDa | |
Mammalian cell |
> 90% as determined by SDS-PAGE. | |
25.75 kDa |