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Recombinant Human Fibroblast Growth Factor 12
Description
Images & Validation
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | The biological activity was determined by its binding ability in a functional ELISA. Immobilized rHuFGF R4/Fc Chimera at 5 μg/mL (100 μL/well) can bind rHuFGF-12 with a linear range of 1.6-100 ng/ml. |
| Molecular Weight | Approximately 20.5 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids. |
| Protein Sequence | MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP IEVCMYREQS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 0.1 EU/ug of rHuFGF-12 as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 1 mM DTT. |
| Disclaimer | For research use only |
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Protocol Information
Recombinant Human Fibroblast Growth Factor 12 (orb1906286)
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