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Catalog Number | orb1906285 |
---|---|
Category | Proteins |
Description | Recombinant Human Fibroblast Growth Factor 16 |
Form/Appearance | Supplied as a 0.2 μm filtered solution in 20 mM Tris-HCl, 1 M NaCl, pH 9.0, with 0.02 % Tween-20, 10 % Glycerol. |
Purity | > 98 % by SDS-PAGE and HPLC analyses. |
MW | Approximately 23.6 kDa, a single non-glycosylated polypeptide chain containing 206 amino acids. |
UniProt ID | O43320 |
Protein Sequence | AEVGGVFASL DWDLHGFSSS LGNVPLADSP GFLNERLGQI EGKLQRGSPT DFAHLKGILR RRQLYCRTGF HLEIFPNGTV HGTRHDHSRF GILEFISLAV GLISIRGVDS GLYLGMNERG ELYGSKKLTR ECVFREQFEE NWYNTYASTL YKHSDSERQY YVALNKDGSP REGYRTKRHQ KFTHFLPRPV DPSKLPSMSR DLFHYR |
Source | Escherichia coli |
Expression System | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x106 IU/mg. |
Endotoxins | Less than 0.1 EU/ug of rHuFGF-16 as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
ELISA | |
> 95% as determined by SDS-PAGE. Ni-NTA chromatography. | |
Escherichia coli |
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