Recombinant Human Fibroblast Growth Factor 19

SKU: orb1906283

Description

Recombinant Human Fibroblast Growth Factor 19

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Assay #1: Fully biologically active when compared to standard. The biological activity is measured by its binding ability in a functional ELISA. Immobilized rHuFGF R4 at 5 μg/ml can bind rHuFGF-19 with a linear range of 3-200 ng/ml.Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 x103 IU/mg.
• Molecular Weight: Approximately 21.8 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids.
• Protein Sequence: MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK
• Purity: > 95 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 1 EU/ug of rHuFGF-19 as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Disclaimer: For research use only

Quick Database Links

UniProt

O95750