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Description
Research Area
Immunology & Inflammation
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human LAG-3-His (Cat#orb2994123) at 5μg/ml (100 μl/well) can bind Human FGL1-mFc (Cat#orb2994023).The ED50 of Recombinant Human FGL1-mFc (Cat#orb2994023) is 30-90 ng/ml. |
| Tag | C-mFc |
| Expression Region | Leu23-Ile312 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 60.6 KDa. Observed: 60-65 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, 5% Trehalose, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Fibrinogen-like protein 1; FGL1; HP-041; Hepassocin; HFREP-1; LFIRE-1
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Predicted: 62.7 KDa. Observed: 60 KDa, reducing conditions
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Human FGL1 Protein, hFc Tag [orb1291005]
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 60.1 kDa after removal of the signal peptide. The apparent molecular mass of hFc-FGL1 is approximately 55-70 kDa due to glycosylation.
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Human FGL1 Protein, His Tag [orb1471797]
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The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 34.8 kDa after removal of the signal peptide. The apparent molecular mass of FGL1-His is approximately 25-35 kDa due to glycosylation.
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10 μg, 50 μg, 100 μg
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Quick Database Links
UniProt
UniProt Details
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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