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Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human ETBR N-ECD (C-Fc) (Cat#orb2992677) at 2 μg/ml (100 μl/well) can bind Anti-Human ETBR mab (Human IgG1).*. *:Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human ETBR mab (Human IgG1) is 38.46 ng/ml. (Regularly tested). |
| Tag | C-Fc |
| Expression Region | Glu27-Lys101 |
| Endotoxins | ＜ 1 EU/μg as determined by LAL test. |
| MW | Predicted: 34.9 KDa. Observed: 40-60 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Endothelin receptor type B; EDNRB; ET-B; ET-BR; Endothelin receptor non-selective type; ETRB
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Protein Handling Guide
Recombinant Human ETBR Protein (orb2992677)
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