Human EpCAM

SKU: orb2994359

Description

Recombinant Human Epithelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln24-Lys265 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human EpCAM (C-Fc)(Cat#orb2994359) at 1μg/ml (100 μl/well) can bind Anti-Human EpCAM Antibody(Human IgG4)*. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Anti-Human EpCAM Antibody is 13.82ng/ml.(Regularly tested).
• Tag: C-Fc
• Molecular Weight: Predicted: 54.5 KDa. Observed: 60-80 KDa, reducing conditions
• Expression Region: Gln24-Lys265
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Epithelial Cell Adhesion Molecule; Ep-CAM; Adenocarcinoma-Associated Antigen; Cell Surface Glycoprotein Trop-1; Epithelial Cell Surface Antigen; Epithelial Glycoprotein 314; EGP314; Major Gastrointestinal Tumor-Associated Protein GA733-2; Tumor-Associated Calcium Signal Transducer 1; CD326; EPCAM; GA733-2; TROP1

Quick Database Links

UniProt

AAH14785.1