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Catalog Number | orb1906302 |
---|---|
Category | Proteins |
Description | Recombinant Human Endostatin |
Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Purity | > 96 % by SDS-PAGE and HPLC analyses. |
MW | Approximately 20.1 kDa, a single non-glycosylated polypeptide chain containing 183 amino acids (C-terminal fragment of Collagen alpha-1(XVIII) chain). |
UniProt ID | P39060 |
Protein Sequence | HSHRDFQPVL HLVALNSPLS GGMRGIRGAD FQCFQQARAV GLAGTFRAFL SSRLQDLYSI VRRADRAAVP IVNLKDELLF PSWEALFSGS EGPLKPGARI FSFDGKDVLR HPTWPQKSVW HGSDPNGRRL TESYCETWRT EAPSATGQAS SLLGGRLLGQ SAASCHHAYI VLCIENSFMT ASK |
Source | Escherichia coli |
Expression System | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inhibiting the FGF basic-dependent proliferation of HUVEC migration is less than 2.0 μg/mL in the presence of Anti-Human Endostatin Polyclonal Antibody. |
Endotoxins | Less than 1 EU/ug of rHuEndostatin as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
46.3 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
26.5 kDa | |
E.coli |
Greater than 96% as determined by SDS-PAGE and HPLC. | |
19.8 kDa | |
E.Coli |
SDS-PAGE | |
Unconjugated | |
> 96% by SDS-PAGE and HPLC analyses. | |
19.8 kDa | |
E. coli |
~20.2 kDa, observed by reducing SDS-PAGE. | |
P. pastoris |
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