Recombinant Human EIF4E Protein

SKU: orb2994583

Description

Recombinant Human Eukaryotic Translation Initiation Factor 4E is produced by our E.coli expression system and the target gene encoding Met1-Val217 is expressed.

Key Properties

• Expression System: E.coli
• Biological Origin: Human
• Tag: Tag free
• Molecular Weight: Predicted: 25.1 KDa. Observed: 25 KDa, reducing conditions
• Expression Region: Met1-Val217
• Purity: SDS-PAGE: Greater than 80% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 95% as determined by SEC-HPLC.(QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20 mM Tris-HCl, 50 mM NaCl, 6% Trehalose, 0.05% Tween80, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; EIF4E; EIF4EL1; EIF4F

Quick Database Links

UniProt

AAH12611.1