Recombinant Human DLL4 Protein

SKU: orb2993713

Description

Recombinant Human Delta-like Protein 4 is produced by our Mammalian expression system and the target gene encoding Ser27-Pro524 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human DLL4 Antibody(Demcizumab,Research Grade) at 1μg/ml (100 μl/well) can bind Recombinant Human DLL4 (C-Fc)(Cat#orb2993713)*. The EC50 of Recombinant Human DLL4 (C-Fc)(Cat#orb2993713) is 3.48 ng/ml(QC verified).*: Biotinylated by NHS-biotin prior to testing.
• Tag: C-Fc
• Expression Region: Ser27-Pro524
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 81.2 KDa. Observed: 85-100 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Tris-HCl, 6% Trehalose, 4% Mannitol, 50mM NaCl, 0.05% Tween80, pH 8.5.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Delta-like protein 4; Drosophila Delta homolog 4; Delta4; DLL4

Quick Database Links

UniProt

Q9NR61