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Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human DLL4 Antibody(Demcizumab,Research Grade) at 1μg/ml (100 μl/well) can bind Recombinant Human DLL4 (C-Fc)(Cat#orb2993713)*. The EC50 of Recombinant Human DLL4 (C-Fc)(Cat#orb2993713) is 3.48 ng/ml(QC verified).*: Biotinylated by NHS-biotin prior to testing. |
| Tag | C-Fc |
| Expression Region | Ser27-Pro524 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 81.2 KDa. Observed: 85-100 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Tris-HCl, 6% Trehalose, 4% Mannitol, 50mM NaCl, 0.05% Tween80, pH 8.5. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Delta-like protein 4; Drosophila Delta homolog 4; Delta4; DLL4
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The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 55.1 kDa after removal of the signal peptide. The apparent molecular mass of DLL4-His is approximately 55-70 kDa due to glycosylation.
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Quick Database Links
UniProt
UniProt Details
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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