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Recombinant Human Cu/Zn Superoxide Dismutase, His
Description
Images & Validation
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The potency per mg was determined by pyrogallol autoxidation method and was found to be more than 1600 U/mg. |
| Molecular Weight | Approximately 39.9 kDa, a homodimer, non-glycosylated polypeptide chain containing 2 x 189 amino acids with Met, Gly and 10 x His at N-terminus. |
| Protein Sequence | MGHHHHHHHH HHSSGHIEGR HMTYARAAAR QARALEATKA VCVLKGDGPV QGIINFEQKE SNGPVKVWGS IKGLTEGLHG FHVHEFGDNT AGCTSAGPHF NPLSRKHGGP KDEERHVGDL GNVTADKDGV ADVSIEDSVI SLSGDHCIIG RTLVVHEKAD DLGKGGNEES TKTGNAGSRL ACGVIGIAQ |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1 EU/ugof rHuCu/Zn SOD, His as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Protocol Information
Recombinant Human Cu/Zn Superoxide Dismutase, His (orb1905972)
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