Human CST1

SKU: orb2995063

Description

Recombinant Human Cystatin SN is produced by our Mammalian expression system and the target gene encoding Trp21-Ser141 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: Enzyme: Measured by its ability to inhibit active Cathepsin L(Cat#orb2993545) cleavage of a fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).The IC50 value is 3.6 nM, as measured under the described conditions. (Regularly tested)
• Tag: C-6His
• Molecular Weight: Predicted: 15.35 KDa. Observed: 17 KDa, reducing conditions
• Expression Region: Trp21-Ser141
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, pH 5.5.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Cystatin-SN; Cystain-SA-I; Cystatin-1; Salivary Cystatin-SA-1; CST1

Quick Database Links

UniProt

P01037